TMB ELISA
TMB ELISA (TMBE) is a highly active and stable 3,3’,5,5’-tetramethylbenzidine / H2O2 formulation intended for
measuring HorseRadish Peroxidase (HRP) probe activity, specifically in Microwell application.
In presence of peroxydase (such as peroxydase-labelled antibodies), the TMBE is oxidized during the enzymatic
degradation of H2O2. The soluble end-product is blue and can be quantified at 650 nm. The use of a stop solution
enhances sensitivity 2-4 fold and produces a yellow end product that can be read at 450 nm.
The stability of the yellow end-product is at least 1 hour.
TMBE substrate is recommended for Microwell application but it is not suitable for applications that require
precipitating reaction.
The stability is at least 3 years from manufacture date onwards if stored at 2-8 °C and protected from light.
| PRODUCT | CODE | VOLUME (ml) |
|---|---|---|
| TMB ELISA (for HRP) | LSE-TMB-10L | 10000 |
| LSE-TMB-1L | 1000 | |
| LSE-TMB-250mL | 250 | |
| LSE-TMB-20mL | 20 |
p-NPP ELISA
para-NitroPhenyl Phosphate (p-NPP) ELISA is a ready-to-use solution that yields after reaction with Phosphatase
(such as Phosphatase-labelled antibodies) a soluble yellow end-product. This end-product can be quantified at 405 nm.
p-NPP substrate is recommended for Microwell application but it is not suitable for applications that require
precipitating reaction.
The p-NPP single component liquid substrate is stable at least 2 years from manufacture date onwards
if stored at 2-8 °C and protected from light.
| PRODUCT | CODE | VOLUME (ml) |
|---|---|---|
| p-NPP ELISA (for Alkaline Phosphatase) | LSE-PNPP-10L | 10000 |
| LSE-PNPP-1L | 1000 | |
| LSE-PNPP-250mL | 250 | |
| LSE-PNPP-50mL | 40 |
TMB Membrane
TMB Membrane (TMBM) contains 3,3’,5,5’-tetramethylbenzidine optimized for use in membrane assays and spot blots.
In presence of peroxydase (such as peroxidase-labelled antibodies), a deep blue color appears at the reaction site.
TMBM substrate is recommended for Membrane application.
The stability is at least 5 years from the date of manufacture onwards if stored at 2-8 °C and protected from light.
| PRODUCT | CODE | VOLUME (ml) |
|---|---|---|
| TMB MEMBRANE (for HRP) | LSM-TMB-10L | 10000 |
| LSM-NBT-1L | 1000 | |
| LSM-TMB-250ml | 250 | |
| LSM-TMB-40ml | 40 |
BCIP/NBT MEMBRANE
BCIP/NBT, the combination of 5-Bromo-4-Chloro-3’-Indolyl-Phosphate (BCIP) and Nitro-Blue
Tetrazolium (NBT), is a highly active and stable formulation intended for measuring Alkaline
Phosphatase (AP) probe activity.
The reducing components which are formed during phosphate hydrolysis of BCIP by AP,
convert NBT to the insoluble purple NBT formazan which deposits on the membrane at the
reaction sites.
The BCIP/NBT substrate is thus particularly useful for colorimetric detection on membrane
immune-assays. Moreover, the black-purple precipitate presents a minimal fading so the
coloration is stable and allows permanent record of the results.
The BCIP/NBT single component liquid substrate is stable for at least 4 years from manufacture date onwards
if stored at 2-8 °C and protected from light.
| PRODUCT | CODE | VOLUME (ml) |
|---|---|---|
| BCIP/NBT MEMBRANE (for Alkaline Phosphatase) | LSM-NBT-10L | 10000 |
| LSM-NBT-1L | 1000 | |
| LSM-NBT-250ml | 250 | |
| LSM-NBT-40ml | 40 |
